Journal: Scientific Reports
Article Title: Akkermansia muciniphila affects colitis by inhibiting ferroptosis signaling pathway
doi: 10.1038/s41598-026-38452-4
Figure Lengend Snippet: A. muciniphila could improve colitis and it was associated with ferroptosis. ( A ) Construction of mouse colitis model (from left to right: weight gain rate; stool shape score; fecal occult blood score). n = 6. ( B ) HE staining of mouse colon tissues. n = 3. ( C ) The number of goblet cells in colon tissues observed by alcian blue staining (black arrows indicate goblet cells). n = 3. ( D ) Transmission electron microscopy of mouse colon tissues (red arrows indicate endoplasmic reticulum, blue arrows indicate mitochondria). n = 3. (E)ELISA of mouse colon tissues. n = 6. ( F ) Western blot detection of mouse colon tissues. n = 3. AKK, Akkermansia muciniphila ; MDA, malondialdehyde; ROS, reactive oxygen species; GSH, glutathione.
Article Snippet: The slices of mice colon tissues were baked, dewaxed, hydrated, and stained with alcian blue staining solution (Servicebio, G1027-100 mL) for 15–30 min.
Techniques: Staining, Transmission Assay, Electron Microscopy, Enzyme-linked Immunosorbent Assay, Western Blot